Nascent peptide‐dependent regulation of protein synthesis

Synthesis of Nascent Protein by Ribosomes in Escherichia Coli.

Nascent peptide regulation of translation.

Translation of mRNA into protein requires the processive decoding of mRNA triplets into amino acids with concurrent formation of peptide bonds. These interdependent events are mediated by the ribosome in association with other cellular components. The complexity of the translation process makes it a likely candidate for regulation at multiple points. Moreover, the existence of small molecules, ...

Nascent peptides that block protein synthesis in bacteria.

Although the ribosome is a very general catalyst, it cannot synthesize all protein sequences equally well. For example, ribosomes stall on the secretion monitor (SecM) leader peptide to regulate expression of a downstream gene. Using a genetic selection in Escherichia coli, we identified additional nascent peptide motifs that stall ribosomes. Kinetic studies show that some nascent peptides dram...

Probing nascent protein folding

Deciphering the mechanisms by which proteins fold remains a substantial challenge for structural biology. In particular, very little is known about cotranslational folding, a process in which folding begins as the nascent protein is still emerging from the ribosome. Recognizing the dearth of methods to study this early folding event, Clark and colleagues present a strategy to stall protein tran...

Protein synthesis regulation by leucine

In vivo and in vitro studies have demonstrated that high protein diets affect both protein synthesis and regulation of several cellular processes. The role of amino acids as substrate for protein synthesis has been established in the literature. However, the mechanism by which these amino acids modulate transcription and regulate the mRNA translation via mTOR-dependent signaling pathway has yet...